Dynamic motions of 1,6-diphenyl-1,3,5-hexatriene in interdigitated C(18):C(10)phosphatidylcholine bilayers
نویسندگان
چکیده
منابع مشابه
Slow motions in bilayers containing anionic phospholipid
Lung surfactant monolayer formation and maintenance requires substantial reorganization of phospholipid assemblies containing up to 10% anionic lipid. Nuclear magnetic resonance was used to study chain order and slow reorientation in bilayer mixtures of dipalmitoylphosphatidylcholine (DPPC) and an anionic phospholipid, dipalmitoylphosphatidylglycerol (DPPG), with one or both lipids chain-perdeu...
متن کاملFluorescence dynamics of diphenyl-1,3,5-hexatriene-labeled phospholipids in bilayer membranes.
A comparative study of the dynamical fluorescence properties of three phosphatidylcholines having a diphenyl-1,3,5-hexatriene (DPH) group attached at different depths from the head group incorporated into membrane vesicles has been carried out. The probes were covalently attached to the sn-2 position of the glycerol part of the phosphatidylcholine via either carboxyl, ethyl or propanoyl links. ...
متن کاملInteraction of the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene with biomembranes.
The relationship between the conditions of membrane labelling by the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH) and its fluorescence parameters was investigated. In the labelling solutions prepared by the usual method, the presence of DPH microcrystals was revealed which led to the lower resultant fluorescence anisotropy values. Lower labelling efficiency was observed with DPH soluti...
متن کاملEffect of cholesterol on membrane microheterogeneity: a study using 1,6-diphenyl-1,3,5-hexatriene fluorescence lifetime distributions.
The effect of cholesterol on microheterogeneity of liposomes obtained from saturated and unsaturated phospholipids was studied by measuring the fluorescence decay of 1,6-diphenyl-1,3,5-hexatriene (DPH). Data obtained by frequency domain fluorometry have been analyzed either by discrete exponential or continuous lifetime distribution approaches. In egg phosphatidylcholine liposomes, the addition...
متن کاملDetection of phospholipid phase separation. A multifrequency phase fluorimetry study of 1,6-diphenyl-1,3,5-hexatriene fluorescence.
Using multifrequency phase and modulation fluorometry and a nonlinear least-squares analysis of lifetime data, we were able to determine the complex decay of 1,6-diphenyl-1,3,5-hexatriene (DPH) in synthetic phospholipid bilayers. Our results showed a monoexponential decay of DPH in the pure isotropic solvents studied, over a wide temperature range, and a double-exponential decay of DPH in phosp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1990
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(90)82439-5